Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases.This resin works well with relatively easy to separate His-tagged proteins.
Low Density Nickel has a lower amount of available groups that usually improve the selectivity/specificity.
REFERENCES:
- Dunne, Matthew et al. “Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins.” Cell Reports 29.5 (2019): 1336-1350.e4. Cell Reports.
| Product Name | Low Density Nickel |
| Cat. No. | 6BCL-QLNi-X |
| Bead Geometry & Size | Spherical,Standard:~ 50-150 µm |
| Crosslinked | Yes |
| Agarose % | 6% |
| Matrix | Stable in all commonly used reagents |
| Binding/Loading Capacity (μmol Me2+/ml gel) | 5-19 |
| Antimicrobial Agent | 20% Ethanol |
| Storage Temperature | 2-8˚C |
ABT Beads 提供一系列优质规则的活化琼脂糖微粒，用于亲和层析、SEC（体积排斥色谱）、固定化和多种纯化。产品主要涉及HIS-TAG融合蛋白的纯化，抗体纯化，GST融合蛋白纯化，酶和抗体的固定化，体积排斥色谱等方面的琼脂糖微粒。及一些分子生物学级电游试剂和用于蛋白纯化用的柱子。
产品列表
AFFINITY CHROMATOGRAPHY
HIS-TAG PURIFICATION HIS-TAG融合蛋白的纯化
• Chelating agarose beads
• Chelating agarose beads NTA
• Chelating Rapid Run™ agarose beads
NEW: 5ml CARTRIDGES!
ANTIBODY PURIFICATION 抗体纯化
• Protein A agarose beads
• Protein A Rapid Run™ agarose beads
GST PURIFICATION GST融合蛋白纯化
• Glutathione agarose beads
ENZYME & ANTIBODY IMMOBILIZATION 酶和抗体的固定化
AMINO GROUPS
• Glyoxal beads
• Glyoxal Rapid Run™ agarose beads
ACIDIC GROUPS
• Aminoethyl beads
• Aminoethyl Rapid Run™ agarose beads
SIZE EXCLUSION CHROMATOGRAPHY 体积排斥色谱
LOW PRESSURE
• Plain and Crosslinked Agarose beads
HIGH PRESSURE
• Rapid Run™ Agarose beads
ELECTROPHORESIS REAGENTS 电游试剂
AGAROSE POWDER
• Agarose LE Molecular Biology Grade
• Agarose HR Molecular Biology Grade
• Agarose GA Electrophoresis Grade
• Agarose LM Molecular Biology Grade
ACCESSORIES 纯化用柱子等辅助产品
• Empty columns
• Empty spin columns
• Mini columns
• Empty cartridges
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