This resin consists of crosslinked agarose derivatized with Nitrilotriacetic acid (NTA) and provides good properties working in native or denaturing conditions. This resin can recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells.
REFERENCES:
- Deneka, Dawid et al. “Structure of a Volume-Regulated Anion Channel of the LRRC8 Family.” Nature 558.7709 (2018): 254–259. Nature.
- Petrenčáková, Martina et al. “Conformational Properties of LOV2 Domain and Its C450A Variant within Broad PH Region.” Biophysical Chemistry 259 (2020): n. pag. Biophysical Chemistry.
- Qian, Yong et al. “A Genetically Encoded Near-Infrared Fluorescent Calcium Ion Indicator.” Nature Methods 16.2 (2019): 171–174. Nature Methods.
- Dunne, Matthew, and Martin J. Loessner. “Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria.” Methods in Molecular Biology. Vol. 1918. Humana Press Inc., 2019. 67–86. Methods in Molecular Biology.
- Huo, Jiangdong et al. “Structural Characterisation of a Nanobody Derived from a Naïve Library That Neutralises SARS-CoV-2.” (2020): n. pag. Web.
| Product Name | Nickel NTA Agarose Resin |
| Cat. No. | 6BCL-NTANi-X |
| Bead Geometry & Size | Spherical,Standard: ~ 50-150 µm |
| Crosslinked | Yes |
| Agarose % | 6% |
| Ligand | Nitrilotriacetic Acid (NTA) |
| Static Binding Capacity | ≥ 50 mg/ ml gel |
| Antimicrobial Agent | 15 % Ethanol of total volume |
| Storage Temperature | 4-8˚C |
ABT Beads 提供一系列优质规则的活化琼脂糖微粒，用于亲和层析、SEC（体积排斥色谱）、固定化和多种纯化。产品主要涉及HIS-TAG融合蛋白的纯化，抗体纯化，GST融合蛋白纯化，酶和抗体的固定化，体积排斥色谱等方面的琼脂糖微粒。及一些分子生物学级电游试剂和用于蛋白纯化用的柱子。
产品列表
AFFINITY CHROMATOGRAPHY
HIS-TAG PURIFICATION HIS-TAG融合蛋白的纯化
• Chelating agarose beads
• Chelating agarose beads NTA
• Chelating Rapid Run™ agarose beads
NEW: 5ml CARTRIDGES!
ANTIBODY PURIFICATION 抗体纯化
• Protein A agarose beads
• Protein A Rapid Run™ agarose beads
GST PURIFICATION GST融合蛋白纯化
• Glutathione agarose beads
ENZYME & ANTIBODY IMMOBILIZATION 酶和抗体的固定化
AMINO GROUPS
• Glyoxal beads
• Glyoxal Rapid Run™ agarose beads
ACIDIC GROUPS
• Aminoethyl beads
• Aminoethyl Rapid Run™ agarose beads
SIZE EXCLUSION CHROMATOGRAPHY 体积排斥色谱
LOW PRESSURE
• Plain and Crosslinked Agarose beads
HIGH PRESSURE
• Rapid Run™ Agarose beads
ELECTROPHORESIS REAGENTS 电游试剂
AGAROSE POWDER
• Agarose LE Molecular Biology Grade
• Agarose HR Molecular Biology Grade
• Agarose GA Electrophoresis Grade
• Agarose LM Molecular Biology Grade
ACCESSORIES 纯化用柱子等辅助产品
• Empty columns
• Empty spin columns
• Mini columns
• Empty cartridges
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