The apoA1 ELISA^PRO kit provides all necessary reagents and solutions required to quantify apoA1 in solution, e.g. in cell culture supernatant, and serum/plasma samples. The kit contains ELISA plates pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and Apo ELISA buffer concentrate. The Apo ELISA buffer enables analysis of serum/plasma samples without interference from heterophilic antibodies. The kit contains pre-coated plates and matched amount of reagents and solutions.

Dilution guidelines for serum/plasma samples can be found here.

Pre-coated ELISA plates save time and reduce assay variability.

Species cross-reactivity

The monoclonal antibodies in this kit cross-react with apoA1 from non-human primates. See the Human/NHP cross-reactivity table.

Analyte Information

ApoA1Apolipoprotein A1 (apoA1, also known as apolipoprotein-A1, apo A1 and apoA-I) is the main protein component of high density lipoprotein particles (HDL). ApoA1 (28 kD) is mainly produced in the liver and exported into the circulation where it acquires lipids and becomes HDL. High level of apoA1 is regarded as protective against atherosclerosis and cardiovascular disease. Normal concentration of apoA1 in human plasma is approximately 0.8-2.2 g/L.